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Analysis of fatty acid hydroxylating cytochrome P450 enzymes
Dr. Viktoria Bruckhoff
Analysis of fatty acid hydroxylating cytochrome P450 enzymes
Based on phylogenetic analysis of Arabidopsis thaliana genomic sequences, uncharacterized fatty acid (FA) hydroxylases were identified. Among them were several cytochrome P450s (CYP). Active site model structures of some CYP enzymes revealed that the hydrophobicity in the heme binding site is very different between enzymes active and inactive towards conversion of FAs. Evidence is given that substrate specificity is conserved among phylogenetically related isoforms of CYP enzymes. Particular residues in the active site pocket play key roles in determining substrate specificity. For this thesis metabolite fingerprinting was employed to analyze the corresponding knock-out mutants for the identified genes in order to identify their in vivo substrate specificity. Furthermore heterologous expression of the desired enzymes in yeast or E. coli and affinity-tag dependent purification were the method of choice to investigate the reaction mechanism and substrate specificity of a certain enzyme. To complete these investigations promoter-GUS-constructs and GFP-fusion proteins allowed elucidation of the tissue-specific and sub-cellular localization.
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39,90 € *
9783863761721
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Analysis of Prokaryotic Fatty Acid Double Bond Hydratases
Anton Volkov
Analysis of Prokaryotic Fatty Acid Double Bond Hydratases
Members of Myosin Cross-Reactive Antigen Family
The present dissertation is focused on the phylogenetic, biochemical, structural and physiological analysis of the hydratases from Streptococcus pyogenes M49 and Lactobacillus acidophilus NCFM. These hydratases belong to myosin cross-reactive antigen (MCRA) protein family for which knowledge on the biochemical and physiological function is scarce. This study provides the first line of evidences on biological significance of this protein family as ubiquitous bacterial enzymes which metabolize highly toxic unsaturated fatty acids to their less toxic and more soluble hydroxy derivatives. Determined in this work de novo crystal structure of Lactobacillus acidophilus NCFM hydratase allows to classify the hydratases to the family of flavin containing amine oxidoreductases and establishes a framework for further biochemical and structural analysis of the rest MCRAs from the pathogenic and commensal bacterial species.
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32,90 € *
9783941274426
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Organically-grown tomato (Lycopersicon esculentum Mill.)
Dr. Mohammed Afrah Eltayeb
Organically-grown tomato (Lycopersicon esculentum Mill.)
Plant infection by Phytophthora infestans and fruit quality
Twenty eight genotypes of tomato were organically cultivated in two locations (Schönhagen and Ellingerode, Germany) in different two years. Plant infection by Phytophthora infestans was evaluated as well as the fruit nutritional and quality parameters (content of lycopene, phenolic compounds, ascorbic acid antioxidant capacity, mineral nutrients and organic compounds). It was also of interest to find a connection between infection and fruit quality.
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32,90 € *
9783941274341
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200 @ listing
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