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Dr. Viktoria Bruckhoff

Analysis of fatty acid hydroxylating cytochrome P450 enzymes

• Georg-August Universität Göttingen • Buch (Gebunden) • Dissertation • Englisch • Softcover (Paperback) • 21,0 x 14,8 cm (DIN A5) • XVI / 186 Seiten • Neu (eingschweißt in Folie)
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  • 9783863761721
  • 978-3-86376-172-1
Based on phylogenetic analysis of Arabidopsis thaliana genomic sequences, uncharacterized fatty... mehr

Based on phylogenetic analysis of Arabidopsis thaliana genomic sequences, uncharacterized fatty acid (FA) hydroxylases were identified. Among them were several cytochrome P450s (CYP). Active site model structures of some CYP enzymes revealed that the hydrophobicity in the heme binding site is very different between enzymes active and inactive towards conversion of FAs. Evidence is given that substrate specificity is conserved among phylogenetically related isoforms of CYP enzymes. Particular residues in the active site pocket play key roles in determining substrate specificity. For this thesis metabolite fingerprinting was employed to analyze the corresponding knock-out mutants for the identified genes in order to identify their in vivo substrate specificity. Furthermore heterologous expression of the desired enzymes in yeast or E. coli and affinity-tag dependent purification were the method of choice to investigate the reaction mechanism and substrate specificity of a certain enzyme. To complete these investigations promoter-GUS-constructs and GFP-fusion proteins allowed elucidation of the tissue-specific and sub-cellular localization.

Titel: Analysis of fatty acid hydroxylating cytochrome P450 enzymes
Autor: Dr. Viktoria Bruckhoff
Hochschule: Georg-August Universität Göttingen
Erschienen: 1. Aufl. 11.12.2015
Fachbereich: Biologie
Produkttyp: Buch (Gebunden)
Produktart: Dissertation
Sprache: Englisch
Einband: Softcover (Paperback)
Maße: 21,0 x 14,8 cm (DIN A5)
Umfang: XVI / 186 Seiten
Zustand: Neu (eingschweißt in Folie)
Keywords: Arabidopsis thaliana, biology, CYP, Cytochrome P450, enzymes, fatty acid, hydroxylation, P450, plant sciences
Details "Analysis of fatty acid hydroxylating cytochrome P450 enzymes"
Titel: Analysis of fatty acid hydroxylating cytochrome P450 enzymes
Autor: Dr. Viktoria Bruckhoff
Hochschule: Georg-August Universität Göttingen
Erschienen: 1. Aufl. 11.12.2015
Fachbereich: Biologie
Produkttyp: Buch (Gebunden)
Produktart: Dissertation
Sprache: Englisch
Einband: Softcover (Paperback)
Maße: 21,0 x 14,8 cm (DIN A5)
Umfang: XVI / 186 Seiten
Zustand: Neu (eingschweißt in Folie)
Keywords: Arabidopsis thaliana, biology, CYP, Cytochrome P450, enzymes, fatty acid, hydroxylation, P450, plant sciences
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